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Faculty Detail    
Name BRADLEY K YODER
 
Campus Address MCLM 688 Zip 0006
Phone 205-934-0994
E-mail byoder@uab.edu
Other websites
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Cell, Developmntl, & Integrative Biology  Cell, Developmntl, & Integrative Biology Professor
Center  Arthritis & Musculoskeletal Diseases Center  Arthritis & Musculoskeletal Diseases Center Professor
Center  Pathology   Cell Adhesion & Matrix Research Center Professor
Center  Center for Aging  COMPREHENSIVE CTR FOR HEALTHY AGING Professor
Center  Center for Metabolic Bone Disease  Center for Metabolic Bone Disease Professor
Center  Comprehensive Cancer Center  Comprehensive Cancer Center Professor
Center  Medicine  Comprehensive Diabetes Ctr Professor
Center  Cystic Fibrosis Research Center  Cystic Fibrosis Research Center Professor
Center  Medicine  Nephrology Res & Trng Ctr Professor
Center  Nutrition Sciences   Nutrition Obesity Res Ctr (NORC) Professor
Center  Vision Sciences  Vision Science Research Center Professor

Graduate Biomedical Sciences Affiliations
Cell, Molecular, & Developmental Biology 
Cellular and Molecular Biology Program 
Genetics and Genomic Sciences 
Hughes Med-Grad Fellowship Program 
Integrative Genetics Graduate Program 
Medical Scientist Training Program 
Pathobiology and Molecular Medicine 

Biographical Sketch 
Dr. Bradley K. Yoder (b. 1966), Professor , completed his undergraduate studies in biochemistry and molecular biology at the University of Maryland Baltimore County (B.S. 1988), and received a Ph.D. in molecular and cellular biology from the University of Maryland in 1993. His postdoctoral studies were performed at Oak Ridge National Laboratory under the guidance of Dr. Rick Woychik where Dr. Yoder was an Alexander Hollaender Distinguished Postdoctoral Fellow. His research over the past two decades has focused on the cellular and molecular mechanisms regulating assembly, maintenance, and function of the primary cilium utilizing complementary approaches in mice, C. elegans, and in cell culture models. Work from his laboratory has utilized genetic screens in C. elegans to identify proteins required for ciliogenesis and cilia mediated signaling activities and how these genes function in pathways (e.g. Daf-2 Insulin/IGF-like pathway) that regulate life span and energy homeostasis. His group has analyzed in mammalian systems how the cilium regulates important developmental pathways and how loss of the cilium causes abnormalities in left-right body axis specification, limb and tooth patterning, skin and hair follicle morphogenesis, and impairs endochondrial bone formation. His group is also providing important fundamental insights into the connection between ciliary dysfunction and cystic kidney disorders, and novel roles for neuronal cilia in the regulation of satiation responses, disruption of which causes obesity and type II diabetes.

Society Memberships
Organization Name Position Held Org Link
American Society of Cell Biology    http://www.ascb.org/ 
American Society of Nephrology    http://www.asn-online.org/ 
National Kidney Foundation    http://www.kidney.org/ 
Society for Developmental Biology    http://www.sdbonline.org/ 

Research/Clinical Interest
Title
Cilia Signaling and Dysfunction in Development and Disease
Description
Cilia are microtubule based structures that can be motile or immotile, the latter being referred to as primary cilia. In contrast to motile cilia, such as those found on epithelia of the trachea, the importance of the primary cilium is relatively undefined despite their presence on most mammalian cells. Cilia are extremely complex organelles which are devoid of ribosomes, thus, proteins required for cilia assembly, maintenance, and signaling must be imported into the cilium. This occurs through a microtubule-based transport system called intraflagellar transport (IFT). Proteins involved in IFT concentrate around the basal body at the base of the cilium and assemble into complexes (IFT particles) which are moved up the cilium by a kinesin and returned by a cytoplasmic dynein. The IFT particle is thought to mediate the transport of cargo into the cilium as well as to deliver signals initiated in the cilium to the cytosol. Although the primary cilium was once thought to be a vestigial organelle, recent studies have uncovered that cilia in mammals are required for viability and that dysfunction of the cilium is associated with a large number of developmental abnormalities and disease phenotypes. This now includes obesity, cystic kidney, liver, and pancreatic diseases, hydrocephalus, skin and hair follicle abnormalities, random left-right body access specification, and skeletal defects. Although studies in mice and humans now indicate that cilia are critically important organelles, the functions of cilia and the pathways in which they are required remains largely unknown. Addressing these issues is the major focus of my groupís research. Our studies utilize comparative approaches in both mouse and C. elegans to investigate four major interrelated themes: 1) What roles do cilia play in regulating signaling pathways? 2) What are the functions of cilia in embryogenesis and tissues physiology in postnatal life? 3) How does cilia dysfunction cause disease and developmental abnormalities? 4) What proteins localize in cilia, how are they targeted to this organelle, and what role do they play in cilia assembly or signaling activities?

Selected Publications 
Publication PUBMEDID
Svetlana V. Masyukova, Marlene E. Winkelbauer, Corey L. Williams, Jay N. Pieczynski, and Bradley K. Yoder Assessing the pathogenic potential of human Nephronophthisis disease-associated NPHP-4 missense mutations in C. elegans. Human Molecular Genetics 20:2942-2954. (2011)   3131040 
Anastasia D. Egorova, Padmini P.S.J. Khedoe, Marie-Jose T.H. Goumans, Bradley K Yoder, Surya M. Nauli, Peter ten Dijke, Robert E. Poelmann, and Beerend P. Hierck. Lack of primary cilia primes shear-induced Endothelial-to-Mesenchymal Transition. Circulation Research, 108(9):1093-101 (2011).  3094764 
P. Darwin Bell, Wayne Fitzgibbon, Antine Stenbit, Sandra Gilley, Amber Houston, Kelli Sas, May Amria, Gene P. Siegal, John Bissler, Mehmet Bilgen, Peter Cheng-te Chou, Lisa Guay-Woodford, Bradley K. Yoder, Courtney J. Haycraft Ryan Reichert, and Brian Siroky. Interactions between Cilia, Hypertrophic Signaling, and Renal Cystogensis. Journal of American Society for Neprhology, (2011). PMCID: PMC3083306  3083306 
Mandy J. Croyle, Jonathan M. Lehman, Amber K. O’Connor, Sunny Y Wong, Erik B. Malarkey, Daniela Iribarne, William E. Dowdle, Trenton R. Schoeb, Zoe M. Verney, Mohammad Athar Edward J. Michaud, Jeremy F. Reiter, and Bradley K. Yoder. Role of Epidermal Primary Cilia in the Homeostasis of Skin and Hair Follicles. Development. 138(9):1675-85 (2011).  3074444 
Corey L. Williams, Chunmei Li, Katarzyna Kida, Peter N. Inglis, Swetha Mohan, Lucie Semenec, Nathan J. Bialas, Rachel Stupay, Nansheng Chen, Oliver E. Blacque*, Bradley K. Yoder*, and Michel R. Leroux*. MKS and NPHP functional modules establish basal body/transition zone-membrane associations and ciliary gate function during ciliogenesis. *Co-corresponding authors. Journal of Cell Biol. 192(6):1023-41. (2011)  3063147 
Edward J Michaud and Bradley K Yoder. Soluble levels of cytosolic tubulin coordinate ciliary length control. Molecular Biol. of the Cell. 22(6):806-16, (2011).   3057705 
Ohazama A, Haycraft CJ, Seppala M, Blackburn J, Ghafoor S, Cobourne M, Martinelli DC, Fan CM, Peterkova R, Lesot H, Yoder BK, Sharpe PT. Primary cilia regulate Shh activity in the control of molar tooth number. Development. 2009 Mar;136(6):897-903. Epub 2009 Feb 11.  2727556 
Kierszenbaum AL, Rivkin E, Tres LL, Yoder BK, Haycraft CJ, Bornens M, Rios RM. GMAP210 and IFT88 are present in the spermatid golgi apparatus and participate in the development of the acrosome-acroplaxome complex, head-tail coupling apparatus and tail. Dev Dyn. 240(3):723-36, (2011).  21337470 
JR Davenport, AJ Watts, VC Roper, MJ Croyle, TV Groen, JM Wyss, TR. Nagy, RA Kesterson3, and BK Yoder. Conditional disruption of intraflagellar transport in adult mice leads to hyperphagia-induced obesity and slow onset cystic kidney disease. Current Biology Sep 18;17(18):1586-94. Epub 2007  2084209 
O'Connor AK, Kesterson RA, Yoder BK. Generating conditional mutants to analyze ciliary functions: the use of cre-lox technology to disrupt cilia in specific organs. Methods Cell Biol. 2009;93:305-30. Epub 2009 Dec 4.  20409823 
Lehman JM, Laag E, Michaud EJ, Yoder BK. An essential role for dermal primary cilia in hair follicle morphogenesis.J Invest Dermatol. 2009 Feb;129(2):438-48. Epub 2008 Nov 6.
 
20362090 
Kesterson RA, Berbari NF, Pasek RC, Yoder BK. Utilization of conditional alleles to study the role of the primary cilium in obesity. Methods Cell Biol. 2009;94:163-79. Epub 2009 Dec 23.
 
20362090  
Williams CL, Masyukova SV, Yoder BK. Normal ciliogenesis requires synergy between the cystic kidney disease genes MKS-3 and NPHP-4.J Am Soc Nephrol. 2010 May;21(5):782-93. Epub 2010 Feb 11.  20150540 
Schneider L, Cammer M, Lehman J, Nielsen SK, Guerra CF, Veland IR, Stock C, Hoffmann EK, Yoder BK, Schwab A, Satir P, Christensen ST.Directional cell migration and chemotaxis in wound healing response to PDGF-AA are coordinated by the primary cilium in fibroblasts. Cell Physiol Biochem. 2010;25(2-3):279-92.  20110689 
Clement CA, Kristensen SG, Møllgård K, Pazour GJ, Yoder BK, Larsen LA, Christensen ST. The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation. J Cell Sci. 2009 Sep 1;122(Pt 17):3070-82. Epub 2009 Aug 4.  19654211  
Clement CA, Kristensen SG, Møllgård K, Pazour GJ, Yoder BK, Larsen LA, Christensen ST. The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation. J Cell Sci. 2009 Sep 1;122(Pt 17):3070-82. Epub 2009 Aug 4.  19654211  
Berbari NF, O'Connor AK, Haycraft CJ, Yoder BK.The primary cilium as a complex signaling center, Curr Biol. 2009 Jul 14;19(13):R526-35.   19602418  
Veland IR, Awan A, Pedersen LB, Yoder BK, Christensen ST. Primary Cilia and Signaling Pathways in Mammalian Development, Health and Disease. Nephron Physiol. 2009 Mar 10;111(3):p39-p53  19276629  
Ohazama A, Haycraft CJ, Seppala M, Blackburn J, Ghafoor S, Cobourne M, Martinelli DC, Fan CM, Peterkova R, Lesot H, Yoder BK, Sharpe PT. Primary cilia regulate Shh activity in the control of molar tooth number. Development. Mar;136(6):897-903. (2009)  19211681  
Sharma N, Berbari NF, Yoder BK. Ciliary dysfunction in developmental abnormalities and diseases. Curr Top Dev Biol. 2008;85:371-427  19147012  
Sharma N, Berbari NF, Yoder BK. Ciliary dysfunction in developmental abnormalities and diseases. Curr Top Dev Biol.;85:371-427. (2008)  19147012  
J Lehman, E Laag, MJ Michaud, BK Yoder. An Essential Role for Dermal Primary Cilia in Hair Follicle Morphogenesis. Journal of Investigative Dermatology, Feb;129(2):438-48. (2009)  18987668  
SK Nielsen, K Møllgård, CA Clement, IR Veland, A Awan, BK Yoder, I Novak, ST Christensen. Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines. Dev Dyn. 237(8):2039-52. 2008  18629868  
ET Johnson, T Nicola, K Roarty, BK Yoder, CJ Haycraft, R Serra. Role for primary cilia in the regulation of mouse ovarian function. Dev Dyn. Aug;237(8):2053-60. (2008)   18629867  
MB Hovater, D Olteanu, EL Hanson, NL Cheng, B Siroky, A Fintha, P Komlosi, W Liu, LM Satlin, PD Bell, BK Yoder, EM Schwiebert. Loss of apical monocilia on collecting duct principal cells impairs ATP secretion across the apical cell surface and ATP-dependent and flow-induced calcium signals. Purinergic Signal. 2008 Jun;4(2):155-70.  18368523  
JM Lehman, EJ Michaud, TR Schoeb, Y Aydin-Son, M Miller, BK Yoder. The Oak Ridge Polycystic Kidney mouse: Modeling ciliopathies of mice and men. Developmental Dynamics 237(8):1960-71. 2008  18366137  
CL Williams, ME Winkelbauer, JC Schafer, EJ Michaud, and BK Yoder. Functional Redundancy of the B9 Proteins and Nephrocystins in C. elegans Ciliogenesis. Mol Biol Cell. 2008  18337471  
PV Tran, CJ Haycraft, TY Besschetnova, A Turbe-Doan, RW Stottmann, BJ Herron, AL Chesebro, H Qiu, PJ Scherz, JV Shah, BK Yoder, and DR Beier. THM1 negatively modulates mouse sonic hedgehog signal transduction and affects retrograde intraflagellar transport in cilia. Nat Genet. 2008  18327258  
C Jones, VC Roper, I Foucher, D Qian, B Banizs, C Petit, BK Yoder, and P Chen. Ciliary proteins link basal body polarization to planar cell polarity regulation. Nat Genet. 40(1):69-77. Epub 2007 Dec 9. (2008)  18066062  
JR Davenport, AJ Watts, VC Roper, MJ Croyle, TV Groen, JM Wyss, TR. Nagy, RA Kesterson3, and BK Yoder. Disruption of intraflagellar transport in adult mice leads to obesity and slow-onset cystic kidney disease. Current Biology Sep 18;17(18):1586-94. Epub 2007 Sep 6. (2007)  17825558  
VV Chizhikov,, JR Davenport, Q Zhang, EK Shih, OA Cabello, J Fuchs, BK Yoder and KJ Millen. Cilia proteins control cerebellar morphogenesis by promoting granule progenitor pool expansion. Journal of Neuroscience Sep 5;27(36):9780-9.(2007)  17804638  
B Song, CJ Haycraft, HS Seo, BK Yoder, and R Serra. Development of the post-natal growth plate requires intraflagellar transport proteins. Dev Biol. May 1;305(1):202-16. [Epub ahead of print] (2007).  17359961  
G Ou, M Koga, OE Blacque, T Murayama, Y Ohshima, JC Schafer, C Li, BK Yoder, MR Leroux, and JM Scholey. Sensory Ciliogenesis in Caenorhabditis elegans: Assignment of IFT components into Distinct Modules Based on Transport and Phenotypic Profiles. Mol Biol Cell. Feb 21; [Epub ahead of print] (2007).  17314406 
SR McGlashan, CJ Haycraft, CG Jensen, BK Yoder, and CA Poole. Articular cartilage and growth plate defects are associated with chondrocyte cytoskeletal abnormalities in Tg737(orpk) mice lacking the primary cilia protein polaris. Matrix Biology, 26(4):234-46 (2006).  17289363  
B Banizs, P Komlosi, MO Bevensee, EM Schwiebert, PD Bell, BK Yoder. Altered intracellular pH regulation and Na+/HCO3- transporter activity in choroid plexus of the cilia defective Tg737orpk mutant mouse. Am J Physiol Cell Physiol. 2006 Dec 20; [Epub ahead of print] (2006)  17182727 
Courtney J. Haycraft, Qihong Zhang, Buer Song, Walker S. Jackson, Peter J. Detloff, Rosa Serra, and Bradley K. Yoder. Intraflagellar Transport is Essential for Endochondral Bone Formation. Development, 134(2):307-16 (2007).   17166921  
Siroky BJ, Ferguson WB, Fuson AL, Xie Y, Fintha A, Komlosi P, Yoder BK, Schwiebert EM, Guay-Woodford LM, Bell PD. Loss of Primary Cilia Results in Deregulated and Unabated Apical Calcium Entry in ARPKD Collecting Duct Cells. Am J Physiol Renal Physiol. 290:F1320-8 (2006)  16396941  
Marlene E. Winkelbauer, Jenny C. Schafer, Courtney J. Haycraft, Peter Swoboda, and Bradley K. Yoder. The C. elegans homologs of Nephrocystin-1 and Nephrocystin-4 are cilia transition zone proteins involved in chemosensory perception. Journal of Cell Science 118(Pt 23):5575-87 (2005)  16291722  
Banizs B, Pike MM, Millican CL, Ferguson WB, Komlosi P, Sheetz J, Bell PD, Schwiebert EM, Yoder BK. Dysfunctional cilia lead to altered ependyma and choroid plexus function, and result in the formation of hydrocephalus. Development 132(23):5329-39. (2005)  16284123  
James R. Davenport and Bradley K. Yoder An incredible decade for the cilium: A look at a once forgotten organelle. American Journal of Physiology 289(6):F1159-69 (2005).  16275743  
Courtney J. Haycraft, Boglarka Banizs, Yesim Aydin-Son, Edward J. Michaud, Bradley K. Yoder. Gli2 and Gli3 localize to cilia and require intraflagellar transport for function. PLOS Genetics PLoS Genet 2005 Oct;1(4):e53. Epub 2005 Oct 28. (2005.  16254602  
Olteanu D, Yoder BK, Liu W, Croyle MJ, Welty EA, Rosborough K, Wyss JM, Bell PD, Guay-Woodford LM, Bevensee MO, Satlin LM, Schwiebert EM. Heightened ENaC-mediated Sodium Absorption in a Murine Polycystic Kidney Disease Model Epithelium Lacking Apical Monocilia. American Journal of Physiology 290: C952-63 (2005).  16207792  
Liu W, Murcia NS, Duan Y, Weinbaum S, Yoder BK, Schwiebert E, Satlin LM. Mechanoregulation of intracellular Ca2+ concentration is attenuated in collecting duct of monocilium-impaired orpk mice. Am J Physiol Renal Physiol. 289(5):F978-88. (2005)  15972389  
AG Kramer-Zucker, F Olale, CJ Haycraft, BK. Yoder, AF Schier, and IA Drummond. Cilia-driving fluid flow is the zebrafish pronephros, brain and Kupffers vesicle is required for normal organogenesis. Development 132: 1907-1921(2005)  15790966  
Qihong Zhang, James. R Davenport., Mandy J. Croyle, C.J. Haycraft, and B.K Yoder. Disruption of IFT results in both exocrine and endocrine abnormalities in the pancreas of Tg737orpk mutant mice. Laboratory Investigation 85:45-64 (2005).  15580285  
Jin Billy Li, Jantje M. Gerdes, Courtney J. Haycraft, Yanli Fan, Tanya M. Teslovich, Helen May-Simera, Haitao Li, , Oliver Blacque, Linya Li, Carmen C. Leitch, Richard Allan Lewis, Jane S. Green, Patrick S. Parfrey, Michel R. Leroux, William S. Davidson, Philip L. Beales, Lisa M. Guay-Woodford, Bradley K. Yoder, Gary D. Stormo, Nicholas Katsanis, and Susan K. Dutcher. Comparative genomic identification of conserved flagellar and basal body proteins that includes a novel gene for Bardet- Biedl syndrome. Cell 117(4):541-52 (2004)  15137946  
Bradley K. Yoder and Susan M. Sell. Jets: a modification to speed flexible oligonucleotide array construction. The Pharmacogenomics Journal 1:163-165 (2001)  11908749 
Xiaoying Hou, Michal Mrug, Bradley K. Yoder, Elliot J. Lefkowitz, Gabriel Kremmidiotis, Peter DEustachio, David R. Beier, and Lisa M. Guay-Woodford. Cystin, a novel cilia-associated protein, is disrupted in the cpk mouse model of polycystic kidney disease. Journal of Clinical Investigation 109:533-540 (2002).  11854326 
Bradley K. Yoder, Albert Tousson, Leigh Millican, John H. Wu, Charles E. Bugg, Jr., James A. Schafer, and Daniel F. Balkovetz. Polaris, a protein disrupted in Oak Ridge Polycystic Kidney mutant mice is required for assembly of renal cilium. American Journal of Physiol. Renal 282: F541-F552 (2002).  11832437 
Courtney J. Haycraft, Peter Swoboda, Patrick D. Taulman, James H. Thomas, Bradley K. Yoder. The C. elegans homologue of the murine cystic kidney disease gene Tg737 functions in a ciliogenic pathway and is disrupted in osm-5 mutant worms. Development 128:1493-1505 (2001)  11290289 

Keywords
Cilia, cystic kidney diseases, obesity, signal transduction, cell fate determination and embryonic patterning, birth defects, mouse and C. elegans models.