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Faculty Detail    
Name MARK OLIVER BEVENSEE
 
Campus Address MCLM 812 Zip 0005
Phone 205-975-9084
E-mail bevensee@uab.edu
Other websites http://138.26.61.118/depts/MEB/SOMResearchFaculty/currentfacultydata.asp?ID=mbevensee
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Cell, Developmntl, & Integrative Biology  Cell, Developmntl, & Integrative Biology Associate Professor
Secondary  Neurobiology  Neurobiology Assistant Professor
Center  Center for Biophysical Sciences/Engineering  Center for Biophysical Sciences/Engineering Associate Professor
Center  Civitan International Research Center  Civitan International Research Center Associate Professor
Center  General Clinical Research Center  Comprehensive Neuroscience Center Associate Professor
Center  Ctr for Glial Bio in Med  Ctr for Glial Bio in Med Associate Professor
Center  Cystic Fibrosis Research Center  Cystic Fibrosis Research Center Associate Professor
Center  Medicine  Nephrology Res & Trng Ctr Associate Professor

Graduate Biomedical Sciences Affiliations
Cell, Molecular, & Developmental Biology 
Integrative Biomedical Sciences 
Integrative Genetics Graduate Program 
Medical Scientist Training Program 
Neuroscience 
Neuroscience Graduate Program 
Neurosciences 
Pathobiology and Molecular Medicine 

Biographical Sketch 
Dr. Mark Bevensee, Associate Professor, received his Ph.D. in 1996 from the laboratory of Dr. Walter F. Boron in the Department of Cellular and Molecular Physiology at Yale University. His postdoctoral work was also done at Yale under the direction of Dr. Boron. In the Fall of 2000, Dr. Bevensee joined the faculty at UAB.

Society Memberships
Organization Name Position Held Org Link
American Physiological Society  Member   
American Society for Biochemistry and Molecular Biology  Member   
American Society for Cell Biology  Member   
Society for Neuroscience  Member   

Research/Clinical Interest
Title
Cellular and Molecular Physiology of Acid-base Transporters and pH Regulation
Description
My laboratory is interested in the cellular and molecular physiology of intracellular pH (pHi) regulation and acid-base transport in tissues such as the brain, heart, and kidney. We are also interested in how acid-base transporters, as well as ion channels such as the epithelial Na+ channel (ENaC) contribute to Na+ hyperabsorption in mouse models of autosomal recessive polycystic kidney disease (ARPKD). Transporters that move acids (e.g., H+) or bases (e.g., bicarbonate or HCO3 ) across the plasma membrane of cells contribute to many cellular processes, including the regulation of pHi, secretion or reabsorption of ions, and modulation of neuronal activity. Some of these transporters include Na-H exchangers, electrogenic and electroneutral Na/HCO3 cotransporters, and Na-dependent and -independent Cl-HCO3 exchangers. In our cellular studies, we use fluorescence approaches with ion-sensitive dyes and electrophysiological techniques (e.g., patch-clamping) to identify and characterize the function and regulation of ion channels and transporters involved in ion movement and acid-base transport across plasma membranes. In our neuroscience research, we are also interested in the effects of pHi changes and transporter activity on neuronal firing and synaptic transmission. The laboratory is also elucidating the molecular nature of Na/bicarbonate cotransporters (NBCs) and related proteins present in brain, heart, and kidney. The laboratory is characterizing structure-function relationships, ion translocation, and regulation of the cloned proteins expressed in either frog oocytes impaled with microelectrodes, or transfected mammalian cells loaded with ion-sensitive dyes. Electrophysiological recordings from excised membrane patches from oocytes enable us to identify and characterize intracellular regulators of NBC activity. The results from our cellular and molecular studies will enhance our understanding of the physiology of pHi regulation and the biophysics of bicarbonate transport, as well as elucidate the contribution of acid-base transporters and ion channels to polycystic kidney disease.

Selected Publications 
Publication PUBMEDID
Current Topics in Membranes. Vol. 73. Exchangers. MO Bevensee, ed. Amsterdam: Academic Press, 2014.   
Bevensee MO, Apkon M & Boron WF (1997b). Intracellular pH regulation in cultured astrocytes from rat hippocampus. II. Electrogenic Na/HCO3 cotransport. J. Gen. Physiol. 110: 467-483.  9379176 
Bevensee MO, Weed RA & Boron WF (1997a). Intracellular pH regulation in cultured astrocytes from rat hippocampus. I. Role of HCO3-. J. Gen. Physiol. 110: 453-465.  9379175 
Grishin AV, Bevensee MO, Modyanov NN, Rajendran V, Boron WF & Caplan MJ (1996). Functional expression of the cDNA encoded by the human ATP1AL1 gene. Am. J. Physiol. Renal Physiol. 271: F539-F551.  8853415 
Bevensee MO, Cummins TR, Haddad GG, Boron WF & Boyarsky G (1996). pH regulation in single CA1 neurons acutely isolated from the hippocampi of immature and mature rats. J. Physiol. 494: 315-328.  8841993 
Zhao J, Hogan EM, Bevensee MO & Boron WF (1995). Out-of-equilibrium CO2/HCO–3 solutions and their use in identifying a novel K+/HCO–3 cotransporter. Nature 374: 636-639.  7715702 
Bevensee MO, Schwiening CJ & Boron WF (1995). Use of BCECF and propidium iodide to assess membrane integrity of acutely isolated CA1 neurons from rat hippocampus. J. Neurosci. Methods 58: 61-75.  7475234 
Thornell IA, Wu J, Liu X & Bevensee MO (2012). PIP2 hydrolysis stimulates electrogenic Na/bicarbonate cotransporter NBCe1-B and -C variants expressed in Xenopus laevis oocytes. J. Physiol. 590: 5993-6011.  22966160 
Olteanu D, Liu X, Liu W, Roper VC, Sharma N, Yoder BK, Satlin LM, Schwiebert EM & Bevensee MO (2012). Increased Na+/H+ exchanger activity on the apical surface of a cilium-deficient cortical collecting duct principal cell model of polycystic kidney disease. Am. J. Physiol.: Cell. Physiol. 302: C1436-C1451.  22301060 
Majumdar D & Bevensee MO (2010). Na-coupled bicarbonate transporters of the Slc4 family in the nervous system: function, localization, and relevance to neurologic function. Neuroscience 171: 951-972.  20884330 
Lee S, Lee HJ, Park HJ, Yang HS, Thornell IM, Bevensee MO & Choi I (2010). Na/bicarbonate cotransporter NBCn1 in the kidney medullary thick ascending limb cell line is upregulated under acidic conditions and enhances ammonium transport. Exp. Physiol. 95:926-937.  20591978 
Wang W, Wu J, Bernard K, Li G, Wang G, Bevensee MO & Kirk KL (2010). ATP-independent CFTR channel gating and allosteric modulation by phosphorylation. Proc. Natl. Acad. Sci., U.S.A. 107:3888-3893.  20133716 
Wu J, McNicholas CM & Bevensee MO (2009). Phosphatidylinositol 4,5-bisphosphate (PIP2) stimulates the electrogenic Na/bicarbonate cotransporter NBCe1-A expressed in Xenopus oocytes. Proc. Natl. Acad. Sci., U.S.A. 106: 14150-14155.  19667194 
Majumdar D, Maunsbach AB, Shacka JJ, Williams JB, Berger UV, Schultz KP, Harkins LE, Boron WF, Roth KA & Bevensee MO (2008). Localization of electrogenic Na/bicarbonate cotransporter NBCe1 variants in rat brain. Neuroscience 155:818-832  18582537 
Bevensee MO & Boron WF (2008). Effects of hypoxia on intracellular-pH regulation in astrocytes cultured from rat hippocampus. Brain Res 1193C:143-152  18190894 
Liu X, Williams JB, Sumpter BR & Bevensee MO (2007). Inhibition of the Na/bicarbonate cotransporter NBCe1-A by diBAC oxonol dyes relative to niflumic acid and a stilbene. J Membr Biol 215:195-204  17578633 
Banizs B, Komlosi P, Bevensee MO, Schwiebert EM, Bell PD & Yoder BK (2007). Altered intracellular pH regulation and Na+/HCO3– transporter activity in choroid plexus of the cilia defective Tg737orpk mutant mouse. Am J Physiol: Cell Physiol 292:C1409-C1416  17182727 
McNicholas-Bevensee CM, DeAndrade KB, Bradley WE, Dell’Italia LJ, Lucchesi PA & Bevensee MO (2006). Activation of gadolinium-sensitive ion channels in cardiomyocytes in early adaptive stages of volume-overload induced heart failure. Cardiovasc. Res. 72: 262-270.  16959228 
McAlear SD & Bevensee MO (2006). A cysteine-scanning mutagenesis study of transmembrane domain 8 of the electrogenic Na/bicarbonate cotransporter NBCe1. J Biol Chem 281: 32417-32427  16936285 
McAlear SD*, Liu X*, Williams JB, McNicholas-Bevensee CM & Bevensee MO (2006). Electrogenic Na/HCO3 cotransporter (NBCe1) variants expressed in Xenopus oocytes: Functional comparison and roles of the amino and carboxy termini. J. Gen. Physiol. 127: 639-658. (* = equal contributors)  16735752 
Olteanu D, Yoder BK, Liu W, Croyle MJ, Welty EA, Rosborough K, Wyss JM, Bell PD, Guay-Woodford LM, Bevensee MO, Satlin LM & Schwiebert EM (2006). Heightened ENaC-mediated sodium absorption in a murine polycystic kidney disease model epithelium lacking apical monocilia. Am. J. Physiol.: Cell. Physiol. 290: C952-C963.  16207792 
Khandoudi N, Albadine J, Robert P, Krief S, Berrebi-Bertrand I, Martin X, Bevensee MO, Boron WF & Bril A (2001). Inhibition of the cardiac electrogenic sodium bicarbonate cotransporter reduces ischemic injury. Cardiovasc. Res. 52: 387-396.  11738055 
Douglas RM, Schmitt BM, Xia Y, Bevensee MO, Biemesderfer D, Boron WF & Haddad GG (2001). Na/H exchangers and Na/HCO3 cotransporter: ontogeny of protein expression in the rat brain. Neuroscience 102: 217-228.  11226686 
Schmitt BM, Berger UV, Douglas R, Bevensee MO, Hediger MA, Haddad GG & Boron WF (2000). Na/HCO3 cotransporters in rat brain: Expression in glia, neurons and choroid plexus. J. Neurosci. 20: 6839-6848.  10995828 
Bevensee MO, Schmitt BM, Choi I, Romero MF & Boron WF (2000). An electrogenic Na+-HCO3- cotransporter (NBC) with a novel COOH-terminus, cloned from rat brain. Am. J. Physiol. 278: C1200-C1211.  10837348 
Bevensee MO, Bashi E & Boron WF (1999). Effect of trace levels of nigericin on intracellular pH and acid-base transport in rat renal mesangial cells. J. Membr. Physiol. 169: 131-139  10341035 
Bevensee MO, Bashi E, Schlue W-R, Boyarsky G & Boron WF (1999). Shrinkage-induced activation of Na-H exchange in rat renal mesangial cells. Am. J. Physiol. 276: C674-C683.  10069995 
Current Topics in Membranes. Vol. 70. Co-transport Systems. MO Bevensee, ed. Amsterdam: Academic Press, 2012.   
Bevensee MO & Boron WF (2013). Control of Intracellular pH. In: Seldin and Giebisch's The Kidney: Physiology and Pathophysiology, Fifth Edition. RJ Alpern, M Caplan, OW Moe, eds. New York: Elsevier, Inc.   
Bevensee MO & Boron WF (2008). Control of Intracellular pH. In: Seldin and Giebisch's The Kidney: Physiology and Pathophysiology, Fourth Edition. RJ Alpern and SC Hebert, eds. New York: Raven Press, Ltd.   
Bevensee MO & Boron WF (1995). Manipulation and regulation of cytosolic pH. In: Methods in Neurosciences. J Kraicer & SJ Dixon, eds. New York: Academic Press 27, 252-273.   
Bevensee MO & Boron WF (1998). pH regulation in mammalian neurons. In: pH and Brain Function. K Kaila & BR Ransom, eds. New York: Willey & Sons, 211-231.   
Bevensee MO & Boron WF (1998). Thermodynamics and physiology of cellular pH regulation. In: pH and Brain Function. K Kaila & BR Ransom, eds. New York: Willey & Sons, 173-194.   
Bevensee MO & Boron WF (1998). Fluorescence indicators. In: pH and Brain Function. K Kaila & BR Ransom, eds. New York: Willey & Sons, 129-151.   
McAlear SD & Bevensee MO (2004). pH regulation in non-neuronal brain cells and interstitial fluid. In: Non-Neuronal Cells of the Nervous System. L Hertz, ed. Amsterdam: Elsevier Science, 707-745.   
Bevensee MO, Alper SL, Aronson PS & Boron WF (2000). Control of intracellular pH. In: The Kidney: Physiology and Pathophysiology, Third Edition. DW Seldin and G Giebisch, eds. New York: Raven Press, Ltd. p. 391-442.   

Keywords
acid-base, bicarbonate, transporter, brain, fluorescence dyes, glia , heart, intracellular pH, microelectrodes, neuron, Xenopus oocyte, neuronal firing, kidney, polycystic kidney disease, epithelial sodium channel