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Faculty Detail     Name MICHELLE L OLSEN   Campus Address MCLM 958A Zip 0005 Phone 205-975-2715 E-mail molsen@uab.edu Other websites

Faculty Appointment(s) Appointment Type Department Division Rank Primary  Cell, Developmntl, & Integrative Biology  Cell, Developmntl, & Integrative Biology Assistant Professor Secondary  Neurobiology  Neurobiology Assistant Professor Center  Ctr for Glial Bio in Med  Ctr for Glial Bio in Med Assistant Professor

Graduate Biomedical Sciences Affiliations Cell, Molecular, & Developmental Biology  Genetics and Genomic Sciences  Neuroscience  Pathobiology and Molecular Medicine

Biographical Sketch  PhD (Neurobiology) University of Alabama at Birmingham 2005

Society Memberships Organization Name Position Held Org Link 2000 - present Society for Neuroscience   Member    2009-present American Association for Cancer Research  Member

Research/Clinical Interest Title Glial potassium channels and glutamate transporters in injury and abnormal development Description The focus of Dr. Olsen's research is to enhance our understanding of the role of astrocytes in brain and spinal cord function. Astrocytes are the most numerous cells in the central nervous system yet the role of astrocytes in injury, particularly pediatric injury, and neurodevelopmental disorders is highly understudied. Her work focuses on two essential functions of astrocytes; buffering of extracellular K+ and glutamate. These functions are thought to be largely mediated by two astrocytic proteins, Kir4.1, an inwardly rectifying potassium channel and excitatory amino acid transporter, GLT-1. These two proteins function to dampen neuronal excitability. Following injury, persistent alterations in the biophysical properties of astrocytes hinder their ability to perform these basic altruistic functions. The resulting dysregulation of extracellular K+ and glutamate are associated with increased neuronal excitability and changes in synaptic physiology and plasticity in the adult. In the developing central nervous system, loss of these functions may profoundly impact neuronal development. Indeed, the immature brain is intrinsically more excitable which may be due in part to lower levels of expression of these two proteins. Surprisingly, little is known regarding the regulation of either protein in normal brain, following injury or during abnormal development. The current research projects span from understanding the regulation of Kir4.1 and GLT-1 gene transcription in pathophysiology, examining protein expression, function and activity, to understanding how the loss of extracellular K+ and glutamate regulation impact neuronal development. To do this work requires a multitude of techniques including functional assays, such as electrophysiology and glutamate uptake assays, confocal and wide field fluorescent imaging, protein biochemistry, and molecular biology. We use tissue and cultured cells derived from several animal models as well as human autopsy and surgical resection tissue.

Selected Publications  Publication PUBMEDID 1. M.L. Olsen, S. Schade, S.A. Lyons, M.D. Amaral, H. Sontheimer. Expression of Voltage-Gated Chloride Channels in Human Glioma Cells, Journal of Neuroscience 23(13)5572-5582, 2003, PMID: 12843258 2. M.L. Olsen, H. Sontheimer. Mislocalization of Kir Channels in Malignant Glia, Glia 46:63-73, 2004, PMCID: 2548404 3. M.L. Olsen, H. Sontheimer.Voltage-Gated Ion Channels in Glial Cells. Neuroglia, 2nd Edition, Oxford University Press, pp112-130, 2004 4. M.L. Olsen, A.K. Weaver, P. Ritch, H. Sontheimer. Modulation of Glioma BK (gBK) Channels via erbB2, Journal of Neuroscience Research 15;81(2):179-89, 2005, PMID: 15948146 5. M.L. Olsen, H Higashimori, S.L. Campbell, J.J. Hablitz and H. Sontheimer. Functional Expression of Kir4.1 Channels in Spinal Cord Astrocytes, Glia 53(5):516-28, 2006, PMCID: 2553202 6. M.L. Olsen, S.L. Campbell and H. Sontheimer. Differential Distribution of Kir4.1 in Spinal Cord Astrocytes Suggests Regional Differences in K+ Homeostasis, Journal of Neurophysiology, 98(2):786-93 , 2007, PMCID: 2040512 7. A.K. Weaver, M.L. Olsen, M. B. McFerrin and H. Sontheimer. BK Channels are Coupled to IP3-receptors via Lipid Rafts: a Novel Mechanism for Coupling [Ca2+]i to Channel Activation, Journal of Biological Chemistry, 26;282(43):31558-68, 2007, PMID: 2227909 8. H. Sontheimer and M.L. Olsen. Whole-Cell Patch Clamp Recordings, Patch-Clamp Analysis: Advanced Techniques, Second Edition, Humana Press, pp35-68, 2008 9. C.M. Habela, M.L. Olsen and H. Sontheimer. ClC3 Is a Critical Regulator of Cell Cycle in Normal and Malignant Cells, Journal of Neuroscience, 28(37):9205-17, 2008, PMID: 18784301 10. M.L. Olsen and H. Sontheimer. Functional Implications for Kir4.1 Channels in Glial Biology: From K+ Buffering to Cell Differentiation, Journal of Neurochemistry, 107, 589–601, 2008 11. M.L. Olsen and H. Sontheimer, Ionic Channels in Glia. Encyclopedia of Neuroscience, Oxford: Academic Press, volume 5, pp. 237-247. 2009 12. M.L. Olsen., S.L. Campbell., C.L. Floyd, H. Sontheimer, Spinal Cord Injury in vivo Causes Wide-Spread, Persistent Loss of Kir4.1 and GLT-1: Potential Benefits of Treatment with 17β-Estradiol, Brain, 133(Pt 4):1013-25, 2010, PMID: 20375134 13. M.L. Olsen, Examining Potassium Channel Function in Astrocytes, Methods in Molecular Biology, Astrocytes: Methods and Protocols, in press, 2010

Keywords brain injury, neurodevelopmental disorders, spinal cord, astrocyte, potassium channel, glutamate transporter, epigenetics

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