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Faculty Detail    
Name JACK R LANCASTER, JR
 
Campus Address BMR2 208 Zip 2180
Phone 205-975-9673
E-mail doctorno@uab.edu
Other websites
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Anesthesiology   Anesthesiology Chair Office Professor
Center  Comprehensive Cancer Center  Comprehensive Cancer Center Professor

Graduate Biomedical Sciences Affiliations
Cancer Biology 
Cell, Molecular, & Developmental Biology 
Integrative Biomedical Sciences 
Pathobiology and Molecular Medicine 

Biographical Sketch 
Dr. Lancaster received his B.Sc. degree in Chemistry in 1970 from the University of Tennessee at Martin, and his Ph.D. degree in Biochemistry from the University of Tennessee medical Units at Memphis. He spent two years’ postdoctoral work with Dr. Peter Hinkle at Cornell University, Ithaca New York and the four years’ postdoctoral work with Dr. Henry Kamin at Duke University Medical Center. In 1980 he was appointed Assistant Professor in the Chemistry and Biochemistry Department at Utah State University, Logan, Utah where he advanced to Associate Professor in 1983. In 1992 he was appointed Associate Professor of Surgery and Pharmacology, The University of Pittsburgh Medical Center. In 1994 he became Associate Professor of Physiology at LSU Health Sciences Center, New Orleans. He became Professor in 1996. He moved to UAB in July 2002 where he is Professor in the Departments of Anesthesiology and Physiology & Biophysics, and is a member of the Center for Free Radical Biology.

Research/Clinical Interest
Title
The Biophysics and Biochemistry of Nitric Oxide
Description
1. Biological roles of nitric oxide and reactive oxygen species: a.Basic studies: Biochemistry and molecular biology of NO and its interactions at the cellular and subcellular levels; cultured cells (hepatocytes, erythrocytes, lymphocytes, mammalian cell lines, bacteria) and isolated proteins (iron-sulfur proteins, electron transferring enzymes) and membranes; Dynamics of NO in blood and tissue: Kinetics, modeling b.Biomedical studies: Roles of NO in injury (inflammation, infection, ischemia/reperfusion) dealing particularly with interactions between NO and reactive oxygen species; Hemoglobin in blood as a major determinant of the biomedical actions of NO; - Biological mechanisms of nitrosation from NO c.Techniques and applications: Electron paramagnetic resonance spectroscopy for in vitro and in vivo detection of NO and its actions; Electrochemical detection of NO; Determination of enzymatic, cellular, organ, and organismal damage from reactive oxygen intermediates; Measurement of cellular enzymatic activities (including the enzymatic synthesis of NO), spectroscopic properties, and metabolism; Methodologies in membrane biochemistry/biophysics; Computer modeling of diffusion/reaction 2.Molecular mechanisms of enzyme-catalyzed electron transfer and biological energy transfer: a.- Conservation of energy coupling in electron transfer and membrane transport of solutes and ions 3.Cellular and biochemical mechanisms of cytokine function: a.- Cytokine production and their effects during immune activation (sepsis, allograft rejection) and oxidative injury during disturbances in oxygen metabolism (ischemia/reperfusion, sepsis, oxidative burst)

Selected Publications 
Publication PUBMEDID
Diffusion-Limited Reaction of Free Nitric Oxide with Erythrocytes Liu, X, Miller, MJS, Joshi, MS, Sadowska-Krowicka, H, Clark, DA, Lancaster, Jr., JR J. Biol. Chem. 1998;273:18709-18713.  9668042 
Accelerated Reaction of Nitric Oxide with O2 Within the Hydrophobic Interior of Biological Membranes Liu, X, Miller, MJS, Joshi, MS, Thomas, DD, Lancaster, Jr., JR Proc. Natl. Acad. Sci. USA 1998;95:2175-2179.  9482858 
Nitric Oxide is Consumed, Rather than Conserved, by Reaction with Oxyhemoglobin under Physiological Conditions. Joshi, MS, Ferguson, Jr., TB, Han, TH, Hyduke, DR, Liao, JC, Rassaf, T, Bryan, N, Feelisch, M, and Lancaster, Jr., JR Proc. Natl. Acad. Sci. USA 2002:99;10341-10346.  12124398 
Nitric Oxide Uptake by Erythrocytes is Primarily Limited by Extracellular Diffusion Liu, X, Samouilov, A, Lancaster, Jr. JR, and Zweier, JL J. Biol. Chem. 2002;277:26194-26190.  12006567 
Reaping of Nitric Oxide by Sickle Cell Disease. Lancaster, JR, Jr. Proc. Natl. Acad. Sci. (Commentary) 2002;99:552-553.  11805311 
Reactive Oxygen Species Mediates Tumor Necrosis Factor-alpha Converting Enzyme-Dependent Ectodomain Shedding Induced by Phorbol Myristate Acetate. Zhang, Z, Oliver, P, Lancaster, JR, Jr., Schwarzenberger, PO, Joshi, MS, Cork, J, and Kolls, JK. FASEB J. 2001;15:303-305.  11156944 
The Biological Lifetime of Nitric Oxide: Implications for the Perivascular Dynamics of NO and O2 Thomas, DD, Liu, X, Kantrow, SP, and Lancaster, JR, Jr. Proc. Natl. Acad. Sci. USA 2001; 98:355-360.  11134509 
Activation of Ectodomain Shedding by Nitric Oxide. Zhang, Z, Kolls, JK, Oliver, P, Good, D, Schwarzenberger,P, Joshi, MS, Ponthier, JL, and Lancaster, Jr, Jr. J. Biol. Chem. 2000:275;15839-15844.  10747938 
Cellular Antioxidant and Prooxidant Actions of Nitric Oxide Joshi, MS, Ponthier, JL, and Lancaster, JR, Jr. Free Rad. Biol. Med. 1999:27;1357-1366  10641730 
The Physical Properties of Nitric Oxide: Determinants of the Dynamics of NO in Tissue. Lancaster, Jr., JR in Nitric Oxide: Biology and Pathobiology Ignarro, LJ, ed. Academic Press, San Diego, CA 2000: pp 209-224   

Keywords
nitric oxide, free radicals, membranes, bioenergetics, mitochondria, electron transfer, EPR spectroscopy, red blood cells, cancer, p53, HIF-1a, computer modeling, diffusion