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Faculty Detail    
Name VICTOR M DARLEY-USMAR
Endowed Professor of Mitochondrial Medicine and Pathology
Director, Center for Free Radical Biology
Vice-Chair of Research Department of Pathology
 
Campus Address BMR2 508 Zip 2180
Phone 205-975-9686
E-mail darley@uab.edu
Other websites
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Pathology   Molecular & Cellular Pathology Professor
Secondary  Anesthesiology   Anesthesiology Chair Office Professor
Secondary  Environmental Health Sciences  Environmental Health Sciences Professor
Secondary  Neurobiology  Neurobiology Professor
Center  Arthritis & Musculoskeletal Diseases Center  Arthritis & Musculoskeletal Diseases Center Professor
Center  Biomedical Engineering  Biomatrix Eng Regen Med (BERM) Ctr Professor
Center  Center for Biophysical Sciences/Engineering  Center for Biophysical Sciences/Engineering Professor
Center  Comprehensive Cancer Center  Comprehensive Cancer Center Professor
Center  Medicine  Comprehensive Diabetes Ctr Professor
Center  General Clinical Research Center  Comprehensive Neuroscience Center Professor
Center  General Clinical Research Center  Ctr for Clinical & Translational Sci Professor
Center  Medicine  Ctr Cardiovasc Bio Professor
Center  Medicine  Nephrology Res & Trng Ctr Professor
Center  Nutrition Sciences   Nutrition Obesity Res Ctr (NORC) Professor

Graduate Biomedical Sciences Affiliations
Cellular and Molecular Biology Program 
Hughes Med-Grad Fellowship Program 
Integrative Biomedical Sciences 
Medical Scientist Training Program 
Molecular and Cellular Pathology Program 
Pathobiology and Molecular Medicine 

Biographical Sketch 
Dr. Darley-Usmar is the UAB Endowed Professor in Mitochondrial Medicine and Pathology. He serves as the Vice Chair of Research for the Department of Pathology and Director of the Center for Free Radical Biology at UAB. His research program focuses on redox cell signaling and molecular bioenergetics in the pathogenesis of human disease. He received his Ph.D. training from Dr. M.T. Wilson at the University of Essex which was to define the structure and function of cytochrome c oxidase. This involved defining whether the monomer or dimer of the enzyme was active and applying the then new techniques of SDS-polyacrylamide gel electrophoresis to membrane proteins. He continued to pursue his interest in mitochondrial proteins with Dr. R. A. Capaldi at the University of Oregon and was one of the first to use Western Blotting to understand the molecular pathology of a mitochondrial myopathy. On returning to the UK after two years as an Assistant Professor in Japan he first became interested in the area of nitric oxide research in industry as a Research Scientist at Wellcome Research Laboratories. Over a ten year period there he worked on the interactions of nitric oxide with reactive oxygen species and mitochondria. Working with Salvador Moncada and Tony Schapira (Royal Free Hospital) he was one of the first to discover that nitric oxide can interact reversibly with cytochrome c oxidase and so control respiration. He left the UK in 1995 to join the Free Radical group at UAB led by Bruce Freeman. Currently he is developing novel methods to assess the measurement of oxidative stress in mitochondria and defining how we determine the mitochondrial response to oxidative stress in the pathologies associated with bioenergetic dysfunction.

Society Memberships
Organization Name Position Held Org Link
American Physiological Society  Member   
Biochemical Society  Member   
Society For Free Radical Biology and Medicine  former President    

Research/Clinical Interest
Title
Mechanisms of Cell Signaling by Mitochondria in Physiology and Disease
Description
The most serious diseases that effect developed nations such as atherosclerosis, neurodegeneration and diabetes are known to involve mitochondrial dysfunction. The challenge is to translate the findings in basic research in mitochondrial function and the pathology of disease to the clinic. To achieve this we are developing new methods to assess bioenergetic dysfunction in human disease and determine its diagnostic potential. The approach we are taking is to develop new methods for defining bioenergetic health in human populations using the cellular mitochondrial function of leukocytes and platelets isolated from human blood.

Selected Publications 
Publication PUBMEDID
Hill B, Benavides G, Lancaster J, Ballinger S, Dell’Italia L, Zhang J, Darley-Usmar VM Integration of cellular bioenergetics with mitochondrial quality control and autophagy. Biological Chemistry. 393: 1485–1512, 2012.   PMCID: PMC3594552 
Mitchell T, Johnson MS, Ouyang X, Chacko BK, Mitra K, Lei X, Gai Y, Moore DR, Barnes S, Zhang J, Koizumi A, Ramanadham S, Darley-Usmar VM (2013) Dysfunctional mitochondrial bioenergetics and oxidative stress in Akita+/Ins2-derived β-cells. American J of Physiology 305(5):E585-99.   PMCID: PMC3761167 
Dodson M, Darley-Usmar VM, Zhang J (2013) Cellular metabolic and autophagic pathways: traffic control by redox signaling. Free Radical Biology and Medicine 63:207-221   PMCID:PMC3729625 
Chacko, B. K., Srivastava, A., Johnson, M. S., Benavides, G. A., Chang, M. J., Ye, Y., Jhala, N., Murphy, M. P., Kalyanaraman, B. and Darley-Usmar, V. M. Mitochondria-targeted ubiquinone (MitoQ) decreases ethanol-dependent micro and macro hepatosteatosis. Hepatology. 54, 153-163 2011.  21520201 
Chacko BK, Reily C, Srivastava A, Johnson MS, Ye Y, Ulasova E, Agarwal A, Zinn KR, Murphy MP, Kalyanaraman B, Darley-Usmar V. Prevention of diabetic nephropathy in Ins2(+/)(AkitaJ) mice by the mitochondria-targeted therapy MitoQ. Biochem J;432:9-19. 2010  20825366 
Hill BG, Dranka BP, Bailey S, Lancaster J, Darley-Usmar V. What part of NO don't you understand? Some answers to the cardinal questions in nitric oxide biology. J Biol Chem, 285, 19699-19704, 2010  20410298 
Perez J, Hill BG, Benevides GA, Dranka BP, Darley-Usmar V. Role of Cellular bioenergetics in smooth muscle cell proliferation induced by platelet-derived growth factor. Biochem J, 2010 428(2):255-67  20331438 
Dranka, B. P., Hill, B. G. and Darley-Usmar, V. M. Mitochondrial reserve capacity in endothelial cells: The impact of nitric oxide and reactive oxygen species. Free Radic Biol Med 48, 905-914, 2010.  20093177 
Hill BG, Higdon AN, Dranka BP, and Darley-Usmar VM. Regulation of vascular smooth muscle cell bioenergetic function by protein glutathiolation. Biochim Biophys Acta, 1797(2):285-95, 2010.  19925774 
Hill BG, Dranka BP, Zou L, Chatham JC, and Darley-Usmar VM. Importance of the bioenergetic reserve capacity in response to cardiomyocyte stress induced by 4-hydroxynonenal. Biochem J 424: 99-107, 2009.  19740075 
Kramer PA, Ravi S, Chacko B, Johnson MS, Darley-Usmar VM. A review of the mitochondrial and glycolytic metabolism in human platelets and leukocytes: Implications for their use as bioenergetic biomarkers. Redox Biol. 2014;2:206-210.   PMCID: PMC3909784. 
Higdon AN, Dranka BP, Hill BG, Oh JY, Johnson MS, Landar A, and Darley-Usmar VM. Methods for imaging and detecting modification of proteins by reactive lipid species. Free Radic Biol Med 47: 201-212, 2009.  19446632 
Chacko BK, Kramer PA, Ravi S, Johnson MS, Hardy RW, Ballinger SW, Darley-Usmar VM (2013) Methods for defining distinct bioenergetic profiles in platelets, lymphocytes, monocytes, and neutrophils, and the oxidative burst from human blood. Lab Invest. 93(6):690-700.   PMCID:PMC3674307 
Oh, J. Y., Giles, N., Landar, A. and Darley-Usmar, V. (2008) Accumulation of 15-deoxy-delta(12,14)-prostaglandin J2 adduct formation with Keap1 over time: effects on potency for intracellular antioxidant defence induction. Biochem J 411, 297-306  18237271 

Keywords
mitochondria, nitric oxide, reactive oxygen species, bioenergetic dysfunciton, translational research