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Faculty Detail    
Name N RAMA KRISHNA
 
Campus Address MCLM 490 Zip 0005
Phone 205-934-5695
E-mail nrk@uab.edu
Other websites
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Biochemistry & Molecular Genetics  Biochemistry & Molecular Genetics Professor
Secondary  NSM Dean's Office (Org Ret)  Physics (Org Ret) Professor
Center  Arthritis & Musculoskeletal Diseases Center  Arthritis & Musculoskeletal Diseases Center Professor
Center  Biomedical Engineering  Biomatrix Eng Regen Med (BERM) Ctr Professor
Center  Pathology   Cell Adhesion & Matrix Research Center Professor
Center  Center for AIDS Research  Center for AIDS Research Professor
Center  Center for Biophysical Sciences/Engineering  Center for Biophysical Sciences/Engineering Professor
Center  Comprehensive Cancer Center  Comprehensive Cancer Center Professor
Center  Medicine  Ctr Cardiovasc Bio (Org Ret) Professor

Graduate Biomedical Sciences Affiliations
Biochemistry and Molecular Genetics Program 
Biochemistry and Structural Biology 
Cancer Biology 
Cellular and Molecular Biology Program 
Medical Scientist Training Program 
Microbiology 

Biographical Sketch 
N. Rama Krishna is a Professor in the Department of Biochemistry and Molecular Genetics. He received his Ph.D. from the Indian Institute of Technology-Kanpur in India. He is the Director of the NMR Core Facility and holds joint appointments in the Comprehensive Cancer Center and the Departments of Chemistry and Physics. He was a Leukemia Society of America Scholar during 1982-87. His research program is supported at various times by grants from the NIH, NSF, American Heart Association, Arthritis Foundation and the Leukemia Society of America.

Research/Clinical Interest
Title
Structural Biology and Biomolecular NMR Spectroscopy
Description
My laboratory is primarily interested in biomolecular NMR spectroscopy and structural biology. Recent work has centered around structure/function investigations and the characterization of motional dynamics in proteins and the development of computational methods and NMR methodologies for macromolecular structure refinement. Some of these investigations involve cross-disciplinary collaborative interactions with other research groups at UAB and elsewhere. Protein Structural Biology: Current work is aimed at understanding the structural biology of the capsid proteins of the HIV-1 virus and other retroviruses. The wild type capsid protein of HIV-1 is particularly challenging to study by 3D/4D-NMR because of its monomer-dimer equilibrium in solution and its tendency to form oligomers. In collaboration with Dr. Prevelige of the Microbiology Department we have been studying a mutant protein that is dimerization-defective and stays as a monomer in solution, and yet is assembly active in both Gag and capsid assembly reactions. Recently we have also determined the detailed solution structure of the monomeric mutant form of the C-terminal domain of the capsid protein by 3D-NMR spectroscopy, and demonstrated that the previously known crystal structures of the dimeric forms of this protein contain dimerization-induced conformational changes in them. Ultimately, a knowledge of the structure of the capsid protein may aid in the development of novel inhibitors that interfere with the HIV-1 life cycle at the level of assembly of the mature and immature capsids. We are also interested in understanding the segmental motions (by 15N relaxation measurements) as well as the slow inter-domain motions in the capsid proteins of HIV-1 and other retroviruses. In a joint collaboration with Dr. McDonald (Pathology) we are also undertaking NMR structural studies to determine the role of calmodulin in Fas-mediated apoptosis of HIV-1 infected cells. A knowledge of the detailed three dimensional structures of calmodulin with cellular targets might lead to the development of calmodulin-antagonists that may have a therapeutic potential. Methodological Development: Our laboratory has an active interest in developing NMR-based structure-refinement procedures. In collaboration with Dr. Istvan Sugar at the Mt. Sinai Medical Center, New York, our laboratory has developed a variable target function based intensity-restrained global optimization procedure (VARTIGO) for refining the three-dimensional structures of proteins using NOESY data. We have developed an alternative method involving Metropolis Simulated Annealing (MSA) refinement of dihedral angles against experimental NOESY intensities. Another major project in our laboratory involved the development of a Complete Relaxation and Conformational Exchange Matrix (CORCEMA) procedure for interpreting the NOESY spectra of interacting molecules such as complexes of reversibly forming ligand-receptor complexes. The CORCEMA theory is very general and is applicable over a wide range of dissociation constants for the complex (weak binding to tight binding). It incorporates explicitly all the pertinent protons of the interacting pair, and can account for the effect of motional dynamics in the complexes (e.g., hinge-bending motions in enzymes) on the NOESY. The CORCEMA program is currently being used in the quantitative analysis of transferred NOESY spectra of ligand/protein complexes at several laboratories in USA and abroad. A copy of the CORCEMA program may be obtained by contacting Dr. Krishna. More recently, we have extended the CORCEMA theory to the saturation transfer difference (STD)-NMR experiment. This theory (CORCEMA-ST) is particularly useful in determining the bound conformations of reversibly binding low molecular weight lead compounds recognized by a target protein. The CORCEMA-ST program developed in our laboratory for analyzing the STD-NMR data quantitatively is currently being used by about 30 different research groups in about 12 countries around the world in their drug-design and developmental work.

Selected Publications 
Publication PUBMEDID
Representative Recent Publications:

Krishna, N. R., and Jayalakshmi, V., “Complete Relaxation and Conformational Exchange Matrix Analysis of STD-NMR Spectra of Ligand-Receptor Complexes”, Invited Review Article. Prorg. NMR Spectrosc. 49:1-25(2006).

Angulo, J., Langpap, B., Blume, A., Biet, T., Meyer, B., Krishna, N. R., Peters, H., Palcic, M.M., Peters, T., Blood group B galactosyl transferase – Insights into substrate binding from NMR experiments. J. Amer. Chem. Soc, 128: 13529-38 (2006).

Krishna, N. R., Jayalakshmi, V., Quantitative Analysis of STD-NMR Spectra of Reversibly Forming Ligand-Receptor Complexes”, “Topics in Current Chemistry: Bioactive Conformations”, Editor, Thomas Peters, Springer, Heidelberg , (2008), Invited Chapter. In press.

Sham, S.W., McDonald, J. M., Micoli, K. J., and Krishna, N. R., Solution structure of a calmodulin-binding domain in the carboxy-terminal region of HIV-1 gp160. AIDS Research and Human Retroviruses, accepted for publication (2008).

Wong, H. C., Shin, R., and Krishna, N. R., Solution structure of a double mutant of the carboxy-terminal dimerization domain of the HIV-1 capsid protein, Biochemistry, 47:2289-2297 (2008).
 
 

Keywords
Proteins, Structural Biology, NMR