Back to Main

Faculty Detail    
Name JIANHUA ZHANG
 
Campus Address BMR2 534 Zip 2180
Phone 205-996-5153
E-mail zhanja@uab.edu
Other websites
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Pathology   Molecular & Cellular Pathology Associate Professor
Secondary  Cell, Developmntl, & Integrative Biology  Cell, Developmntl, & Integrative Biology Associate Professor
Secondary  Neurobiology  Neurobiology Assistant Professor
Center  Center for Aging  COMPREHENSIVE CTR FOR HEALTHY AGING Associate Professor
Center  Civitan International Research Center  Civitan International Research Center Associate Professor
Center  General Clinical Research Center  Comprehensive Neuroscience Center Associate Professor
Center  Neurology   Ctr Neurodegeneration & Exp Ther (CNET) Associate Professor

Graduate Biomedical Sciences Affiliations
Cell, Molecular, & Developmental Biology 
Cellular and Molecular Biology Program 
Integrative Genetics Graduate Program 
Medical Scientist Training Program 
Molecular and Cellular Pathology Program 
Neuroscience 
Neuroscience Graduate Program 
Pathobiology and Molecular Medicine 

Biographical Sketch 
Dr. Zhang graduated from University of Science and Technology of China. She received her PhD from University of Texas Southwestern Medical Center at Dallas. She then worked as a postdoctoral associate in the Whitehead Institute for Biomedical Research, and as a Research Assistant Professor in University of Cincinnati College of Medicine. She joined UAB in 2005.

Society Memberships
Organization Name Position Held Org Link
American Physiology Society  member   
Association for Neuron and Disease (AND)  member   
Birmingham Chinese Professor Association (BCPA)  president   
Chinese Biologic Investigator Society (Ray Wu society)  member   
Society for Free Radical Biology and Medicine (SFRBM)  council member   
Society for Neuroscience (SFN)  member   
Society of Chinese Bioscientists in America (SCBA)  member   

Research/Clinical Interest
Title
Mechanisms and regulation of autophagy-lysosomal pathway in health and diseases
Description
Our long-term research interests are to understand the cellular and molecular mechanisms responsible for autophagy, cellular bioenergetics, oxidative stress and cell death, in the context of human diseases. We use transgenic and conditional knockout mouse models, cell differentiation models to dissect mechanisms and pathways. Autophagy regulation is important for healthy aging, combating neurodegenerative, cardiovascular, liver and metabolic diseases. My laboratory currently constitutes 2 PhD earning graduate students, 2 postdocs and 1 research associate. We are currently funded by NINDS.

Selected Publications 
Publication PUBMEDID
Sheshadri N, Catanzaro J, Bott A, Sun Y, Ullman E, Chen E, Pan J-A, Wu S, Crawford H, Zhang J, Zong WX (2014) SCCA1/SerpinB3 promotes epithelial-mesenchymal transition and oncogenic transformation via unfolded protein response and IL-6 signaling. Cancer Research in press   
Wani W, Boyer-Guittaut M, Dodson M, Chatham J, Darley-Usmar V, Zhang J (2014) Regulation of autophagy by protein post-translational modification. Laboratory Investigation In press   
Bioenergetic adaptation in response to autophagy regulators during rotenone exposure.
Giordano S, Dodson M, Ravi S, Redmann M, Ouyang X, Darley Usmar VM, Zhang J.
J Neurochem. 2014 Jul 31. doi: 10.1111/jnc.12844. [Epub ahead of print] 
25081478 
Redox regulation of antioxidants, autophagy, and the response to stress: Implications for electrophile therapeutics
AL Levonen, BG Hill, E Kansanen, J Zhang, VM Darley-Usmar
Free Radical Biology and Medicine 71, 196-207 
24681256 
Chacko B, Kramer P, Ravi S, Benavides G, Mitchell T, Dranka BP, Ferrick D, Singal A, Ballinger S, Bailey SM, Hardy R, Zhang J, Zhi D, Darley-Usmar VM (2014) The Bioenergetic Health Index: A new concept in translational research. Clinical Science   24895057 
Redmann M, Dodson M, Boyer-Guittaut M, Darley-Usmar VM, Zhang J (2014) Mitophagy mechanisms and role in human diseases. International Journal of Biochemistry and Cell biology   24842106 
The role of GABARAPL1 (GEC1) in autophagic flux and mitochondrial quality control in MDA-MB-436 breast cancer cells  24879149 
Giordano S, Darley-Usmar V, Zhang J. Autophagy as an essential cellular antioxidant pathway in neurodegenerative disease. 2: 82–90.   24494187 
Hengartner CJ, Thompson CM, Zhang J, Chao DM, Liao SM, Koleske AJ, Okamura S, Young RA.Association of an activator with an RNA polymerase II holoenzyme.Genes Dev. 1995 Apr 15;9(8):897-910.  7774808 
Zhang, J., Liao, S.-M., Jeffrey, D.A., Koleske, A.J., Thompson, C.M., Chao, D.M., Viljoen, M., van Vuuren, H.J.J. & Young, R.A. (1995). A kinase-cyclin pair in the RNA polymerase II holoenzyme. Nature 374:193-196 (co-first author)  7877695 
Zhang, J., Liu, X., Scherer, D.C., Van Kaer, L., Wang, X. & Xu, M. (1998). Resistance to DNA fragmentation and chromatin condensation in mice lacking the DNA fragmentation factor 45. Proc. Natl. Acad. Sci. USA 95: 12480-12485 with commentary by Susan Cory: “Cell death throes”  9770511  
Zhang, J., Wang, X., Bove, K. & Xu, M. (1999). DNA fragmentation factor 45 deficient cells are more resistant to apoptosis and exhibit different dying morphology than wild-type control cells. J. Biol. Chem. 274:37450-37454  10601318  
Zhang, J. & Xu, M. (2002). Apoptotic DNA degradation and tissue homeostasis. Trends Cell Biol 12:84-89  11849972  
Zhang, J., Zhang, D., McQuade, J.S., Behbehani, M., Tsien, J. & Xu, M. (2002). C-fos regulates neuronal excitability and survival. Nature Genet 30:416-420  11925568 
Zhang, J., McQuade, J.S., Vorhees, C. & Xu, M. (2002). Hippocampal expression of c-fos is not essential for spatial learning. Synapse 46:91-99   12211087 
Zhang, J., Dong, M., Li, L., Fan, Y., Pathrie, P., Dong, J., Olivares-Villagom, D., Lou, D., Wells, J., Van Kaer, L., Wang, X. & Xu, M. (2003). Endonuclease G is required for early embryogenesis and normal apoptosis in mice. Proc. Natl. Acad. Sci. USA 100:15782-15787   14663139  
Zhang, L., Lou, D., Jiao, H., Zhang, D., Wang, X., Xia, Y., Zhang, J. & Xu, M. (2004) Cocaine-induced intracellular signaling and gene expression are oppositely regulated by the dopamine D1 and D3 receptors. J. Neurosci. 24 3344-3354  15056714  
Zhang, D., Zhang, L., Zhang, Q., Tang, Y., Lou, D., Sharp, F.R., Zhang, J. & Xu, M. (2005) Gene expression changes induced by repeated cocaine administration through the dopamine D1 receptors. Neuropsychopharmacology 30, 1443-1454.  15770241  
Jianhua Zhang, Lu Zhang, Hongyuan Jiao, Qi Zhang, Dongsheng Zhang, Danwen Lou, Jonathan L. Katz, and Ming Xu (2006) c-Fos facilitates the acquisition and extinction of cocaine-induced persistent changes. Journal of Neuroscience 26: 13287-13296  17182779 
Shacka J, Lu J, Xie Z, Uchiyama Y, Roth K, Zhang J (2007) “Kainic Acid Induces Early and Transient Autophagic Stress in Mouse Hippocampus” Neuroscience letter 414, 57-60  17223264  
Qianjun Li, Huashi Li, Bradley J. Blitvich and Jianhua Zhang (2007) The Aedes albopictus inhibitor of Apoptosis 1 gene protects vertebrate cells from bluetongue virus-induced apoptosis Insect Molecular Biology. 16:93-105  17257212  
Shacka J, Roth K, Zhang J (2008) The Autophagy-Lysosomal Degradation Pathway: Role in Neurodegenerative Disease and Therapy Frontiers in bioscience (review) 13:718-36  17981582 
Ness J, Harvey C, W Jason, Roth K, Carroll S, Zhang, J (2007) Differential activation of c-Fos and caspase-3 in hippocampal neuron subpopulations following neonatal hypoxia-ischemia. J Neuroscience Res 86:1115-24  18030677  
SB Caine, M Thomsen, KI Gabriel, JS Berkowitz, LH Gold, GF Koob, S Tonegawa, J Zhang, M Xu (2007) Lack of Self-Administration of Cocaine in Dopamine D1 Receptor Knockout Mice. J Neurosci 27:13140-13150  18045908 
Qiao L, Hamamichi S, Caldwell KA, Caldwell GA, Yacoubian TA, Wilson S, Xie ZL, Speake LD, Parks R, Crabtree D, Liang Q, Crimmins S, Schneider L, Uchiyama Y, Iwatsubo T, Zhou Y, Peng L, Lu YM, Standaert DG. Walls KC, Shacka JJ, Roth KA, Zhang J (2008) Lysosomal enzyme cathepsin D protects against neuronal alpha-synuclein aggregation and toxicity. Molecular Brain 1:17  19021916 
Qiao L, Zhang J (2009) Effect of inhibition of lysosomal functions on proteasomal activity. Neurosci Lett 456:15-19.  19429125  
Walls KC, Ghosh AP, Franklin AV, Klocke BJ, Ballestas M, Shacka JJ, Zhang J, Roth KA. Lysosome dysfunction triggers Atg7-dependent neural apoptosis. J Biol Chem. 2010 Apr 2;285(14):10497-507. Epub 2010 Feb 1.  20123985  
Schneider L, Zhang J (2010) Lysosomal function in macromolecular homeostasis and bioenergetics in Parkinson's disease Molecular Neurodegeneration. Molecular Neurodegeneration 5:14.  20388210 
Jegga A, Schneider L, Ouyang X, Zhang, J (2011) Systems biology of the autophagy-lysosomal pathway. Autophagy 7:5, 1-13.   21293178 
Liang Q, Ouyang X, Schneider L, Zhang J. Reduction of mutant huntingtin accumulation and toxicity by lysosomal cathepsins D and B in neurons. Mol Neurodegener. 2011 Jun 1;6:37.  21631942 
Dranka BP, Benavides GA, Diers AR, Giordano S, Zelickson BR, Reily C, Zou L, Chatham JC, Hill BG, Zhang J, Landar A, Darley-Usmar VM. Assessing bioenergetic function in response to oxidative stress by metabolic profiling.
Free Radic Biol Med. 2011 Aug 16. [Epub ahead of print] 
21872656 
Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress.Schneider L, Giordano S, Zelickson BR, Johnson M, Benavides G, Ouyang X, Fineberg N, Darley-Usmar VM, Zhang J. (2011)Free Radic Biol Med. 51:2007-2017. PMCID: PMC3208787  21945098 
Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling.Lee J, Giordano S, Zhang J.Biochem J. 2012 Jan 15;441(2):523-40.PMID:22187934PMC3258656  22187934 
Higdon AN, Benavides GA, Chacko B, Ouyang X, Johnson MS, Landar A, Zhang J, Darley-Usmar VM (2012) Hemin causes mitochondrial dysfunction in endothelial cells through promoting lipid peroxidation: the protective role of autophagy. American Journal of Physiology 302(7):H1394-409.  22245770 
Jaber N, Dou Z, Chen JS, Catanzaro J, Jiang YP, Ballou LM, Selinger E, Ouyang X, Lin R, Zhang J, Zong WX (2012) The Class III PI3K Vps34 plays an essential role in autophagy and in the heart and liver function. PNAS. 109:2003-2008. Co-corresponding author. PMCID: PMC3277541  22308354 
Jaber N, Dou Z, Lin RZ, Zhang J, Zong WX (2012) Mammalian PIK3C3/VPS34: The key to autophagic processing in liver and heart. Autophagy 8:4, 1–2  22498475 
Cummins TD, Higdon AN, Kramer PA, Chacko B, Riggs DW, Salabei JK, Dell’Italia LJ, Zhang J, Darley-Usmar VM, Hill BG (2012) Utilization of fluorescent probes to identify subcellular proteomes and processes regulated by lipid peroxidation products FRBM Jun;59:56-68  22954622 
Klionsky et al (Zhang J as one of 1270 co-authors) (2012) Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy in Higher Eukaryotes. Autophagy 8:4, 445-544  22966490 
Giordano S, Jisun Lee, Darley-Usmar VM, Zhang J (2012) Distinct effects of rotenone, 1-methyl-4-phenylpyridinium and 6-hydroxydopamine on cellular bioenergetics and cell death. PlosOne 7(9):e44610. PMCID:PMC3435291  22970265 
Hill B, Benavides G, Lancaster J, Ballinger S, Dell’Italia L, Zhang J, Darley-Usmar VM (2012) Integration of cellular bioenergetics with mitochondrial quality control and autophagy. Biological Chemistry 393:1485-1512  23092819 
Errami Y, Naura AS, Kim H, Ju J, Suzuki Y, El-Bahrawy, AH, Ghonim MA, Hemeida RA, Mansy MS, Zhang J, Xu M, Boulares AH (2013) Apoptotic DNA fragmentation may be a cooperative activity between caspase-activated DNase and the PARP-regulated DNas1L3, an ER-localized endonuclease that translocates to the nucleus during apoptosis. J Biol Chem. 2013 Feb 1;288(5):3460-8.  23229555 
Mitchell T, Bailey SM, Ballinger SW, Zhang J, Darley-Usmar VM (2013) Convergent mechanisms for mitochondrial dysfunction in metabolic disease; insights from mitochondrial therapeutics. Biochemical Society Transactions 41:127-133  23356271 
Errami Y, Brim H Oumouna-Benachour K, Oumouna M, Naura AS, Kim H, Ju J, Kim JG, Ashktorab H, Fallon K, Xu M, Zhang J, Del Valle L, Boulares HA (2013) ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability". PlosOne 2013;8(2):e57871.  23451280 
Parekh VV, Wu L, Boyd KL, Williams, JA, Gaddy JA, Olivares-Villagomez D, Cover TL, Zong WZ, Zhang J, Van Kaer L (2013) Impaired autophagy, defective T cell homeostasis and a wasting syndrome in mice with a T cell-specific deletion of Vps34. Journal of Immunity 2013 May 15;190(10):5086-101.  23596309 
Le Grand JN, Bon K, Fraichard A, Zhang J, Jouvenot M, Risold P-Y, Boyer-Guittaut M, Delage-Mourroux R (2012) Specific distribution of the autophagic protein GABARAPL1/GEC1 in the developing and adult mouse brain and identification of neuronal populations expressing GABARAPL1/GEC1. PlosOne 2013 May 15;8(5):e63133  23690988 
Dodson M, Darley-Usmar V, Zhang J.Cellular Metabolic and Autophagic Pathways: Traffic Control by Redox Signaling.
Free Radic Biol Med. 2013 May 20. pii: S0891-5849(13)00224-4.  
23702245 
Biochem J. 2013 Jun 17. [Epub ahead of print]Bioenergetic and autophagic control by Sirt3 in response to nutrient deprivation in mouse embryonic fibroblasts.Liang Q, Benavides GA, Vasilopoulos A, Gius D, Darley-Usmar V, Zhang J.  23767918 
Mitchell T, Johnson MS, Ouyang X, Chacko BK, Mitra K, Lei X, Gai Y, Moore DR, Barnes S, Zhang J, Koizumi A, Ramanadham S, Darley-Usmar VM.Dysfunctional mitochondrial bioenergetics and oxidative stress in Akita+/Ins2-derived beta-cells. Am J Physiol Endocrinol Metab. 2013 Jul 2. [Epub ahead of print]  23820623 
Zhang J (2013) Autophagy and mitophagy in cellular damage control. Redox Biology. 1:19-23  23946931 
Benavides GA, Liang Q, Dodson M, Darley-Usmar V, Zhang J. Inhibition of autophagy and glycolysis by nitric oxide during hypoxia-reoxygenation impairs cellular bioenergetics and promotes cell death in primary neurons.Free Radic Biol Med. 2013 Sep 19. pii: S0891-5849(13)00609-6. doi: 10.1016/j.  24056030 

Keywords
Autophagy, Parkinson's Disease, Huntington's disease, signal transduction, mouse genetic engineering, cell and molecular biology, lysosomes, mitochondria, oxidative stress