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Faculty Detail    
Name TODD JASON GREEN
 
Campus Address CBSE 128 Zip 4400
Phone 205-975-0591
E-mail tgreen@uab.edu
Other websites
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Microbiology  Microbiology Assistant Professor

Graduate Biomedical Sciences Affiliations
Biochemistry and Structural Biology 

Biographical Sketch 
I was introduced to scientific work as an under-graduate researcher where I studied natural products chemistry. Here, I isolated naturally occurring compounds from plant material in search of potential therapeutic agents. Compounds that were active against various bacteria as well as compounds that were cytotoxic to cancer cell lines were discovered during this research period. Upon beginning my graduate career in the laboratory of Dr. Ming Luo, I began to study viruses with an emphasis in virus structure. This work has continued since I took my faculty position at UAB. Specifically, I have been involved with studying the structure of negative strand RNA viruses. Our work with the nucleocapsid of vesicular stomatitis virus (VSV) has been instrumental in showing how RNA is encapsidated and sequestered in the nucleocapsid as well as how the nucleocapsid is assembled. In addition, we have solved crystal structures of the VSV phosphoprotein P showing how P multimerizes. We have continued this research solving the structure of N with bound P protein. This has demonstrated how P interacts with the nucleocapsid and aided in formulating hypotheses about how the viral polymerase may be delivered to the active template for transcription and replication. The active template for both of these processes is the N protein with the encapsidated genome. Our research to date has contributed greatly to the field of structural virology. It has also aided in developing new experiments that continue within our lab.

Research/Clinical Interest
Title
Viral Transcription and Replication, Capsid Structure
Description
My current research interests include crystallographic studies of proteins involved in polynucleotide synthesis for negative strand RNA viruses. These efforts have included: structure determination of the nucleocapsid of VSV encapsidated with various sequences of RNA, as well as complexes of the nucleocapsid with the polymerase co-factor, P.

Selected Publications 
Publication PUBMEDID
Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus. Green TJ, Rowse M, Tsao J, Kang J, Ge P, Zhou ZH, Luo M. J Virol. 2011 Mar;85(6):2714-22. doi: 10.1128/JVI.01927-10. Epub 2010 Dec 22.  21177817 
Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity. Meng G, Zhao Y, Bai X, Liu Y, Green TJ, Luo M, Zheng X. J Biol Chem. 2010 Dec 17;285(51):39898-904. doi: 10.1074/jbc.M110.130781. Epub 2010 Aug 19.  20724479 
Cryo-EM model of the bullet-shaped vesicular stomatitis virus. Ge P, Tsao J, Schein S, Green TJ, Luo M, Zhou ZH. Science. 2010 Feb 5;327(5966):689-93. doi: 10.1126/science.1181766.  20133572 
Characterization of a mumps virus nucleocapsidlike particle. Cox R, Green TJ, Qiu S, Kang J, Tsao J, Prevelige PE, He B, Luo M. J Virol. 2009 Nov;83(21):11402-6. doi: 10.1128/JVI.00504-09. Epub 2009 Aug 19.  19692473 
Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P. Green TJ, Luo M. Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11713-8. doi: 10.1073/pnas.0903228106. Epub 2009 Jul 1.  19571006 
Crystallization and preliminary X-ray crystallographic studies on SI-CLP, a novel human Glyco_18 domain-containing protein. Meng G, Bai X, Green TJ, Luo M, Zheng X. Protein Pept Lett. 2009;16(3):336-8.  19275751 
Structure of the vesicular stomatitis virus nucleoprotein-RNA complex.Green TJ, Zhang X, Wertz GW, Luo M. Science. 2006 Jul 21;313(5785):357-60. Epub 2006 Jun 15.

 
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